Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine A1-Acid Glycoprotein (also known as A1-AGP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine A1-AGP antibody. A1-AGP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine A1-AGP Antibody is added and binds to A1-AGP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated A1-AGP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine A1-AGP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm